Composite

Part:BBa_K1783002

Designed by: Chun Mun Loke, Adam Wahab, Kimia Abtahi, Iowis Zhu   Group: iGEM15_UMaryland   (2015-07-18)

Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)

Produces unstable RFP under a constitutive promoter. This mutant RFP originated from coral (Part:BBa_E1010). This BioBrick includes a LVA-ssrA degradation tag that decreases the lifespan of this protein in E. coli. This LVA tag is reported to degrade RFP at a rate of -0.018 per minute, yielding a half-life of approximately one hour.

Usage and Biology

The rapid degradation of unstable RFP due to the LVA tag makes this BioBrick an excellent candidate for real-time assessment of plasmid presence or absence through fluorescence measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None